Effects of Surface Chemistry Interaction on Primary Neural Stem Cell Neurosphere Responses

The characteristics of a material’s surface are extremely important when considering their interactions with biological species. Despite chemistry playing critical role in mediating the responses cells, there remains no single rule which dictates absolute performance; this is particularly challenging response differing cell types to range materials. Here, we highlight functional behavior neural stem cells presented as neurospheres, respect alkane-based self-assembled monolayers presenting different groups: OH, CO2H, NH2, phenyl, CH3, SH, and laminin. influence chemical cues was examined terms neurosphere spreading on each these defined surfaces (cell adhesion migration capacity) neuronal versus glial marker expression. Measurements were made over time series 3, 5, 7 days, showing dynamic nature initial observed after seeding. While OH an excellent platform for migration, larger proportions expressing β3-tubulin found SH- laminin-coated surfaces. Axonal elongation be initially similar all neurite lengths having wider spread predominantly NH2- laminin-presenting A generalized trend could not correlate cellular wettability, lipophilicity (log P), or charge/ionizability (pKa). These results potential direct primary contact New biomaterials control specific culture vitro will streamline up-scale manufacture therapies, enrichment required populations resulting from material interaction.